WebCardiomyocyte cell cycle arrest is initiated in the first few days after birth in mice [4].During this early postnatal period, cell cycle inhibitory factors are induced while cardiomyocyte proliferative factors are repressed [8].To assess expression of Btg1 and Btg2 (Btg1/2) in early and late postnatal mouse ventricles, C57BL/6 wildtype mouse hearts were … WebFeb 27, 2024 · Intrathoracic injection of a high dose of the vector led to approximately 35% correction at the transcript ... The lifespan of homozygous mice, which usually die …
Intrathoracic Injection for the Study of Adult Zebrafish Heart
WebFeb 27, 2024 · Background Epidemiological data suggests that obstructive sleep apnea (OSA) is associated with increased cancer incidence and mortality. We investigate the effects of cyclical intermittent hypoxia (CIH), akin to the underlying pathophysiology of OSA, on lung cancer progression and metastatic profile in a mouse model. Methods … WebMethods: A549 cells (5×10 6 mL-1) were suspended and inoculated into the right lung of BALB/c nude mice via intrathoracic injection. Nude mice were scanned three times … gesture drawing definition art
Maximizing response to intratumoral immunotherapy in mice by
WebOct 11, 2011 · Background Preclinical models of non-small cell lung cancer (NSCLC) require better clinical relevance to study disease mechanisms and innovative therapeutics. We … 1–1: The PC-9 cell line (formerly known as PC-14; ACACC90071810; Sigma-Aldrich, St. Louis, MO) was cultured in RPMI (Dutscher, Brumath, France) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Dutscher) and 2 mM L-glutamine (Dutscher). The A549 cell line (CCL-185™, ATCC, Manassas, VA) … See more 2- : The following procedure involving animals was performed according to institutional guidelines (Agreement APAFIS # 8887; regional ethics committee of Brittany; France). Balb/c nude (BALB/cAnNRj … See more 4–1: NSCLC Luc+ cells were carefully resuspended by pipetting before being drawn into a syringe with a 25 G needle (1 × 106cells in 200 μL / … See more 3–1: NSCLC Luc+ cells were carefully resuspended by pipetting before being drawn into a syringe with a 25 G needle (1 × 106cells in 50 μL / mouse). 3–2: The mouse was anesthetized using an isoflurane chamber … See more 5–1: NSCLC Luc+ cells were carefully resuspended by pipetting before being drawn into a syringe with a 25 G needle (1 × 106cells in 50 μL / mouse). 5–2: The mouse was … See more WebMar 5, 2024 · aegypti were infected by intrathoracic injection with 10 3 PFU of ZIKV, and after 7 or 10 days allowed to feed on naïve mice. (A) Experimental workflow for the ZIKV … gesture drawing reference generator