Fl83b cells

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August undefined, 2024

WebApr 25, 2024 · Briefly, FL83B cells (~ 18 × 10 6) were collected by centrifugation and sonicated in cold diluted assay buffer. The supernatant was removed, and cells were re-suspended in cold diluted assay buffer. BHB standard wells and sample wells were prepared as per the protocol. The reaction was initiated by adding developer solution. WebElk-3 siRNA at 20 nM was transfected into TGF-β1-treated FL83B cells (A) or AML12 cells (B). At 48 hours posttransfection, the cells were harvested and lysed. Western blot …

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WebJul 1, 2014 · FL83B cell morphology before and after DEHP exposure at different concentrations are shown in Fig. 1. With the increase of DEHP concentration, cells tend to shrink. Afterward, the cell lost its ability to adhere on the plate of the dish; thus, it was found floating in the media. Beside shrinkage, cells also showed decrease in cell density. WebJan 15, 2024 · FL83B cells were induced as fatty liver cell model by 0.5 mM oleic acid for 48 h, and treated with various concentration of fucoxanthin for 24 h. The results demonstrated that fucoxanthin significantly suppressed lipid accumulation and decreased lipid peroxidation in hepatocytes. ray fischer cape may nj

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WebJul 13, 2007 · FL83B cells, derived from mouse liver, were treated with insulin and membrane translocation of GRK2 was determined using immunofluoresecence and … WebJul 1, 2024 · 2.2. Cell line and treatment. FL83B cells purchased from the Bioresource Collection and Research Center (BCRC, Taiwan). Cells were cultured in F12 medium (Invitrogen-Gibco™, Paisley, Scotland) added with 10% FBS, 2 mM glutamine, 1% penicillin and streptomycin at 37 °C in a humidified atmosphere containing 5% CO 2.Cells … Web(F) Lipid content analysis of FL83B cells treated with 400 mM OA and different CLH concentrations by using Nile red stain. The data are given as mean ± SEM (n ¼ 6). ray fischer and gal gadot against joss whedon

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GRK2 Negatively Regulates Glycogen Synthesis in Mouse Liver FL83B Cells

WebA mouse HCC cell line (Hepa1-6) and a mouse hepatocyte line (FL83B) were used to elucidate the mechanism Cite 6th Dec, 2024 Mohamed Elkablawy Taibah University and … WebFAM83A-H are newly identified oncogenes characterised by a conserved DUF1669 domain. FAM83B can substitute for Ras to promote malignant transformation. Ablation of … simple teleprompter windowsWebJul 13, 2024 · FL83B cells were incubated in 400 µM palmitate diluted in growth medium for 24 h. One percent BSA diluted in growth medium was used as control. Moreover, gene knockout was achieved by the CRISPR-Cas9 system, using non-targeting agRNA (control) and Tcf4 or Mafk sgRNA. simple television for elderly

"WebApr 1, 1973 · The FL83B cells were not disrupted to determine whether VLDL was present intracellularly. In summary, our data show that the FL83B mouse liver cell line grows in a … " - Fl83b cells

Fl83b cells

WebFL83B is a hepatocyte cell line derived in 1969 by Charity Waymouth at The Jackson Laboratory, Bar Harbor, Maine from a normal liver taken from a 15-17 day old fetal …

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Web2.3. FL83B cell culture First, the culture method of FL83B cells was slightly modiﬁed from the American Type Culture Collection (ATCC) [18]. The short- and long-term impacts of CLHs on the survivability of FL83B cells in fetal bovine serum (FBS) containing culture sys-tem were determined (Fig. 1A), which could monitor WebDec 1, 2024 · Among the subfractions, EAF5-5 markedly enhanced glucose uptake in TNF- α -treated FL83B cells. The possible bioactive compounds of the EAF5-5 fraction that were identified by the HPLC assay include syringic acid, p -coumaric acid, and cirsimaritin. The bioactive compound with the best effect of increasing glucose uptake was p -coumaric …

Web"KYO-1 Cells;急变期的慢性粒白血病活化克隆细胞 STR基因图谱 GD-C60408935 背景信息：详见相关文献介绍产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支) 【细胞培养中原虫的污染情况总结】原虫：培养液可轻微浑浊，显微镜下那些细小的点状 WebDec 9, 2024 · FL83B cells were harvested at the exponential growth phase, prepared into a suspension of approximately 1 × 10 6 cells/mL, and 600 μL of this suspension was added to the lower chamber. FL83B cells were stimulated with 40 ng/mL TNF-α for 2 h in model group (+ TNF-α group). FL83B cells were stimulated with an equal volume of PBS for 2 …

WebMay 8, 2024 · The effects of a triterpene purified from bitter melon was investigated against TNF-α-induced inflammation via AMP-activated protein kinase in FL83B cells. This compound suppressed the TNF-α-induced expression of inflammatory markers, including inducible NO synthase, p65 subunit of NF-κB, TNF-α, and IL-1β (Cheng et al., 2012). WebMay 21, 2024 · Abstract. FAM83B has been recently identified as an oncogene, but its role in thyroid cancers (TC) is still unclear. We examined the expression of FAM83B and its …

WebApr 1, 1973 · The FL83B cells are morphologically similar to rat liver parenchymal cells [29], to the granular epithelial cells seen on primary explantation of rat liver [30], and to the secondary liver parenchymal cell line obtained by cloning a suspension of rat liver cells [2]. The question of transformation must be addressed in trying to determine the ...

WebIn another experiment, FL83B cells were challenged with oleic acid to induce lipid accumulation and treated with various concentrations of fisetin. Results: NAFLD mice treated with fisetin had decreased body weight and epididymal adipose tissue weight compared to NAFLD mice. Fisetin treatment also reduced liver lipid droplet and … ray firthWebOriginal magnification: 100×. (C) FL83B cells were transfected with HA-Daxx and then treated with or without TGF-β for 24 h. Western blotting analysis of Daxx, E-cadherin and Vimentin expression. Relative expression was normalized to β-actin expression as a reference. The graph shows quantitative densitometry from Western blotting. ray firth artistWebFeb 6, 2013 · FL83B mouse hepatocytes were treated with tumor necrosis factor-α (TNF-α) to induce insulin resistance to investigate the effect of a wax apple aqueous extract (WAE) in insulin-resistant mouse hepatocytes. The uptake of 2-[N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl)amino]-2-deoxyglucose (2 NBDG), a fluorescent d-glucose derivative, was performed, … simple telescope drawingWebThe FL83B cells were treated with insulin for 5 or 10 min. GRK2 was labeled with anti-GRK2 antibody and Cy3-conjugated secondary antibody and the cells were observed under the microscope. Insulin treatment increased the translocation of GRK2 from cytosol to the plasma membrane ( Fig. 1A ). ray fischer charleston scWebThe short-and long-term impacts of CLHs on the survivability of FL83B cells in fetal bovine serum (FBS) containing culture system were determined (Fig. 1A), which could monitor cellular morphology ... ray fish and chickenWebElk-3 siRNA at 20 nM was transfected into TGF-β1-treated FL83B cells (A) or AML12 cells (B). At 48 hours posttransfection, the cells were harvested and lysed. Western blot … simple television speakersWebHowever, whether reversibility exists in mesenchymal stromal cell (MSC)-derived hepatocytes (dHeps) remains elusive. In this study, we find that hepatogenic differentiation (HD) of MSCs is a reversible process and is modulated by DNA methyltransferases (DNMTs). DNMTs are regulated by transforming growth factor β1 (TGFβ1), which in turn ... ray fishburn