WebDec 25, 2013 · 我现有deae Sepharose Fast Flow（GE的）填料100ml，进行蛋白质纯化，想问选多粗和多高的柱子进行纯化比较好？ 还是上述100ml填料，假如我的填料装入柱子后，填料的高是20cm，直径是2.5cm，十分钟大概能通过多少ml液体？ WebHiTrap DEAE FF is prepacked with DEAE Sepharose Fast Flow, and is a weak anion exchanger for small-scale protein purifications as well as screening of binding and elutions conditions. Convenient and affordable for fast, easy ion exchange separations either alone or connected in series. The industry standard for ion exchange chromatography.
How much DEAE sepharose to be added in a column?
WebDec 2, 2013 · A new in vitro anti-tumor polypeptide, coded as J2-C3, was isolated from Arca inflata Reeve and purified by diethyl-aminoethanol (DEAE)-sepharose Fast Flow anion exchange and phenyl sepharose CL-4B hydrophobic chromatography. J2-C3 was identified to be a homogeneous compound by native polyacrylamide gel electrophoresis (Native … WebIt is composed of crosslinked 6% agarose beads, with diethylaminoethyl (DEAE) weak anion exchange groups. DEAE Sepharose Fast Flow has high chemical stability, allowing well … bvp m80 modernizacija
HiTrap® DEAE Fast Flow Cytiva 17-5055-01, pack of 5 × 1 mL
WebNote: For DEAE and Q Sepharose Fast Flow, the starting buffer must be at least one pH unit above the pI of the molecule to be bound. For CM and SP Sepharose Fast Flow, … WebDEAE Sepharose ® Fast Flow. Cytiva 17-0709-01, pack of 500 mL. View Price and Availability. Sigma-Aldrich. DCL6B100. DEAE–Sepharose ... WebDEAE Sepharose ® Fast Flow. Cytiva 17-0709-01, pack of 500 mL. View Price and Availability. ... SP Sepharose ® Fast Flow, aqueous ethanol suspension, 45-165 μm (wet), exclusion limit ~4,000,000 Da. View Price and Availability. Properties. Quality Level. 200. bead size. 45-165 μm. pore size bvp program login