WebFiGure 1. A serial dilution series of recombinant GFP analyzed on Wes. A serial dilution series from 2 ng/mL to 0.125 ng/mL was created in 0.1X Sample Buffer. The peak area is plotted for each concentration, and a line of fit was calculated. GFP was detected using a goat anti-GFP antibody. WebMonoclonal Antibody for studying GFP jellyfish. Cited in 630 publications. Validated for Western Blotting, Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific …
Anti-GFP antibody (ab13970) Abcam
WebThe cells were labeled with GFP Polyclonal Antibody (Product # A10262) at 1:100 dilution and Histone H3 Rabbit Polyclonal Antibody (Product # 711055) at 0.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius … WebApr 6, 2024 · Infectious titers were determined from the number of cells infected with rNDV GFP or canine IFNγ (cIFNγ) by flow cytometry analysis (Beckman Coulter, Inc., United States) using mouse B16 cells. A total of 10 5 B16 cells were infected with serial tenfold dilutions of rNDV-GFP or canine IFNγ (cIFNγ) in 24-well plates (four wells per dilution ... soham hedgehog guardians
Anti-GFP antibody (GTX13970) GeneTex
WebChicken IgY (H+L) Secondary Antibody (A16054) in WB. Knockdown of Calreticulin was achieved by transfecting MCF 7 cells with Calreticulin specific validated siRNAs (Silencer® select Product # s114). Western blot analysis (Fig 1) was performed using whole cell extracts from the Calreticulin knockdown cells (lane 3), non-specific scrambled siRNA ... WebWestern Blotting Protocol. For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween ® 20 at 4°C with gentle shaking, overnight.. NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.. A. Solutions and Reagents Web126 rows · Antibodies were analyzed by western blot analysis (1:5000 dilution) and immunohistochemistry (1:500 dilution) using transgenic mice expressing the GFP gene product. Western blots were performed using … soham heritage \u0026 art centre