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WebNational Center for Biotechnology Information WebNov 18, 2008 · The construct pFull-MBs has been generated by the self-ligation of the vector pFull digested with Mlu1 and BssH2, both fragment ends were blunted by fill-in reactions in presence of deoxinucleotides and the Klenow enzyme. To generate pFull-Bg, the DNA fragment of about 1.8 kb obtained from pGL3 Basic digested with Bgl2 and Xba1 was … WebProduct Source. An E. coli strain that carries the BssHII gene from Bacillus stearothermophilus H3 (N. Welker). This product is related to the following categories: … twilight new movie